Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching
Abstract Real-time 4 post backdrop stand visualisation of chick embryo development occurring inside an opaque eggshell is a major goal of developmental biology.This goal was partially achieved when 3-day-old embryos recovered from fertilised shelled eggs, preincubated in a conventional incubator, hatched after the egg contents had been transferred